The scalene muscles are three paired muscles (anterior, middle and posterior) located in the lateral aspect of the neck. They form part of the floor of the posterior triangle of the neck. The scalenes act as accessory muscles of respiration and perform flexion at the neck. In this article, we shall look at the anatomy of the scalene muscles – their attachments, innervation and clinical correlations. By TeachMeSeries Ltd (2025) Fig 1The anterior, middle and posterior scalene muscles of the neck Pro Feature - 3D Model You've Discovered a Pro Feature Access our 3D Model Library Explore, cut, dissect, annotate and manipulate our 3D models to visualise anatomy in a dynamic, interactive way. Learn More Scalene Muscles Anterior Scalene The anterior scalene muscle is located deep to the sternocleidomastoid on the lateral aspect of the neck. Attachments: Originates from the anterior tubercles of the transverse processes of C3-C6. It attaches onto the scalene tubercle (on the inner border of the first rib). Function: Elevation of the first rib. Ipsilateral contraction causes ipsilateral lateral flexion of the neck, and bilateral contraction causes anterior flexion of the neck. Innervation: Anterior rami of C5-C6. Middle Scalene The middle scalene is the largest and longest of the three scalene muscles. It has several long, thin muscle bellies arising from the cervical spine, which converge into one large belly that inserts into the first rib. Attachments: Originates from the posterior tubercles of the transverse processes of C2-C7. It attaches onto the superior aspect of the 1st rib. Function: Elevation of the first rib. Ipsilateral contraction causes ipsilateral lateral flexion of the neck. Innervation: Anterior rami of C3-C8. Posterior Scalene The posterior scalene is the smallest and deepest of the scalene muscles. Unlike the anterior and middle scalene muscles, it inserts into the second rib. Attachments: Originates from the posterior tubercles of the transverse processes of C5-C7. It attaches onto the second rib. Function: Elevation of the second rib, and ipsilateral lateral flexion of the neck. Innervation: Anterior rami of C6-C8. Pro Feature - Dissection Images The root of the neck. The trunks of the brachial plexus are visible emerging between the anterior and middle scalene muscles. The root of the neck. The trunks of the brachial plexus are visible emerging between the anterior and middle scalene muscles. You've Discovered a Pro Feature Access our Dissection Image Library Enhance your understanding with high-resolution dissection images showcasing real-life anatomy. Learn More Anatomical Relationships The scalene muscles are an important part of the anatomy of the neck, with several important structures located between and around them. The brachial plexus and subclavian artery pass between the anterior and middle scalene muscles. This provides an important anatomical landmark in anaesthetics for performing an interscalene block. The subclavian vein and phrenic nerve pass anteriorly to the anterior scalene – the subclavian vein courses horizontally across it, while the phrenic nerve runs vertically down the muscle. The subclavian artery is located posterior to the anterior scalene. By TeachMeSeries Ltd (2025) Fig 2Proximal portion of the brachial plexus, in the neck. Clinical Relevance of the Scalene Muscles Interscalene block The brachial plexus courses between the bellies of the anterior scalene and middle scalene muscles. In upper limb surgery, the brachial plexus can be infiltrated with local anaesthetic to avoid the use of a general anaesthetic – known as an interscalene block. To do this, local anaesthetic is injected between these muscles at the level of the cricoid cartilage. Accessory Muscles of Respiration The scalene muscles collectively act to elevate the first and second ribs, and in doing so they increase the intrathoracic volume. In patients with respiratory distress, the scalene muscles may be used as ‘accessory muscles of respiration’ to aid with breathing. By increasing intrathoracic volume, the patient can ventilate their lungs more effectively. However, they are not required in the respiration of a healthy individual, and so the use of accessory muscles is an important clinical sign of respiratory distress. Do you think you’re ready? Take the quiz below Pro Feature - Quiz The Scalene Muscles Question 1 of 3 Submitting... Skip Next Rate question: You scored 0% Skipped: 0/3 1800 More Questions Available Upgrade to TeachMeAnatomy Pro Challenge yourself with over 1800 multiple-choice questions to reinforce learning Learn More Print Article Rate This Article