The Testes and Epididymis

Original Author: Oliver Jones
Last Updated: January 22, 2018
Revisions: 31

The testes and epididymis are paired structures, located within the scrotum. The testes are the site of sperm production and hormone synthesis, while the epididymis has a role in the storage of sperm.

In this article, we shall look at the anatomy of the testes and epididymis – their structure, vasculature, innervation and clinical correlations.

Anatomical Position

The testes are located within the scrotum, with the epididymis situated on the posterolateral aspect of each testicle. Commonly, the left testicle lies lower than the right. They are suspended from the abdomen by the spermatic cord – collection of vessels, nerves and ducts that supply the testes.

Originally, the testes are located on the posterior abdominal wall. During embryonic development they descend down the abdomen, and through the inguinal canal to reach the scrotum. They carry their neurovascular and lymphatic supply with them.

Fig 1.0 - The testes and epididymis, surrounded by the tunica vaginalis.

Fig 1.0 – The testes and epididymis, surrounded by the tunica vaginalis.

Anatomical Structure

The testes have an ellipsoid shape. They consist of a series of lobules, each containing seminiferous tubules supported by interstitial tissue.

Spermatozoa are produced in the seminiferous tubules. The developing sperm travels through the tubules, collecting in the rete testes. Ducts known as efferent tubules transport the sperm from the rete testes to the epididymis for storage.

Fig 1.2 - Structure of the testes and epididymis.

Fig 1.1 – Structure of the testes and epididymis.

The testes have two coverings. The tunica vaginalis is situated externally, covering the anterior surface and sides of each testicle. It is derived from abdominal peritoneum during development. The tunica albuginea is a fibrous capsule that encloses the testes. It penetrates into the parenchyma of each testicle, dividing it into lobules.

The epididymis consists of a single heavily coiled duct. It can be divided into three parts; head, body and tail.

  • Head – The most proximal part of the epididymis. It is formed by the efferent tubules of the testes, which transport sperm from the testes to the epididymis.
  • Body – Formed by the heavily coiled duct of the epididymis.
  • Tail – The most distal part of the epididymis. It marks the origin of the vas deferens, which transports sperm to the urethra for ejaculation.


The testes and epididymis receive innervation from the testicular plexus – a network of nerves derived from the renal and aortic plexi.

They receive autonomic and sensory fibres.

Vascular Supply

Arterial supply to the testes and epididymis is via the paired testicular arteries, which arise directly from the abdominal aorta. They descend down the abdomen, and pass into the scrotum via the inguinal canal, contained within the spermatic cord.

Venous drainage is achieved via the paired testicular veins. They are formed from the pampiniform plexus in the scrotum – a network of veins wrapped around the testicular artery. In the abdomen, the left testicular vein drains into the left renal vein, while the right testicular vein drains directly into the inferior vena cava.

Fig 1.2 - The pampiniform plexus. Note how it surrounds the testicular artery.

Fig 1.2 – The pampiniform plexus. Note how it surrounds the testicular artery.


The lymphatic drainage is to the paired lumbar and para-aortic nodes, located at the L1 vertebral level. This is in contrast to the scrotum, which drains into the nearby superficial inguinal nodes.

Clinical Relevance: Enlargement of the Scrotum

The scrotal sac is very distensible, and will enlarge in response to the size of its contents. There are a number of causes of scrotal swelling – here are the most common:

Fig 1.2 - Venogram of the varicocoele.

Fig 1.3 – Venogram of the varicocoele.

  • Inguinal hernia – where the contents of the abdominal cavity protrude into the scrotum, via the inguinal canal.
  • Hydrocoele – a collection of serous fluid within the tunica vaginalis. It is most commonly due to a failure of the processus vaginalis to close.
  • Haematocoele – a collection of blood in the tunica vaginalis. It can be distinguished from a hydrocoele by transillumination (where a light is applied to the testicular swelling). Due to the dense nature of blood, light is unable to pass through.
  • Varicocoele – gross dilation of the veins draining the testes.The left testicle is more commonly affected, as the left testicular vein drains into a smaller vessel, the left renal vein, at a perpendicular angle. A large varicocoele can look and feel like a bag of worms within the scrotum.
  • Epididymitis – inflammation of the epididymis, usually caused by bacterial or viral infection.

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Question 1 / 6
Where are the testes originally located during embryonic development?


Question 2 / 6
Which of the following describes the body of the epididymis?


Question 3 / 6
Which label indicates the seminiferous tubules?


Question 4 / 6
What is the innervation of the testes and epididymis?


Question 5 / 6
What structure does the left testicular vein drain into?


Question 6 / 6
Which of the following correctly describes varicocoele?


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